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3D Printing in Medicine: Fountain of Youth? BioPrinting Analysis

I attended a wonderful talk put on by GABA (German American Business Association) on 10/11/17 about how 3D bioprinting’s ability to print precise, complex and individual features has opened new roads for medicine.  The speakers shared the current efforts to create organs and tissue and what the future has in store.  The presentation including a diverse panel of six speakers, Dr. Jenny Chen, MD, Dr. Melanie Matheu, Dr. Mayasari Lim, Dr. Nabeel Cajee, DDS FICOI, Tom Anderton, who each explained the impact of bioprinting in industry, academia, education/global outreach, and patents.

Jenny Chen

Founder/CEO of 3DHEALS is trained as a neuroradiologist, and her company is focusing on curating healthcare 3D printing ecosystem. Her main interests include medical education, 3D printing in the healthcare sector, and artificial intelligence. She is also a current adjunct clinical faculty in the radiology department at Stanford Healthcare.  She was also the event’s moderator and lead each Q&A section with each speaker as well as an open panel Q&A at the end.

Dr. Mayasari Lim

Founder and CEO of SE3D, a startup focused on next-generation bioprinting tools for accelerating research and education in the biomedical and biotech fields. Previously, she was a professor in Bioengineering at Nanyang Technological University (NTU), a top engineering university in Singapore. Her research expertise included stem cell engineering, bioprocess design, bioprinting and tissue engineering.  She is greatly passionate about training next generation minds for the future of bioprinting. She currently teaches at the Fung Institute for Engineering  Leadership at UC Berkeley. Her background obtained her Ph.D. degree in Chemical Engineering at Imperial College London and her BSc in Chemical Engineering at UC Berkeley.  She believes the future outlook of 3D bioprinting will be used for creating personalized medicine, drug screening, tissue replacement and future organ transplant. She hopes to accelerate the future of bioprinting by offering educators an affordable bioprinters, coupled with comprehensive curriculum and laboratory teaching materials to trigger research and exploration.

She began the event with describing the three main ways of achieving bioprinting: laser-assisted bioprinting, inkjet bioprinting, and microextrusion bioprinting.  Below are videos showing the various processes.

Laser-assisted bioprinting

Inkjet bioprinting

Microextrusion bioprinting

 

She pointed out the obstacles and tradeoffs in the bioprinting process; mechanical properties versus the biological interaction of the cells printed as well as the post-processing of bioprinting.  The process of placing down the cells was the easy part, keeping the cells alive and maintaining their intended purpose in the structure was the difficult part.  There are chemical, physiological, biological, mechanical, and cell to cell interaction that must be monitored.  These factors can be influenced in a controlled environment by adjusting the temperature, pH, humidity, and other factors as well.  This monitoring is critical to understanding how bioprinted structures can be suitable for the real world environment.   She also explained the obstacle of iPS reprogramming factors to make sure the cells had specific tasks to perform when they were printed since it is hard to program the cells to do what they do.

 

Dr. Matheu

Co-founded Prellis Biologics in October 2016, with the mission to create fully vascularized human tissues and organs from transplantation. She orchestrated a cross-pollution solution of laser technology at the center of her Ph.D. thesis with biology to creating the tiny blood vessels, known as microvasculature, necessary for tissue engineering applications. Without microvasculature, organs are starved of oxygen and nutrients.  Dr. Matheu brings her multi-disciplinary experience in specialized laser microscopy, cell biology, physiology, and biophysics to build microvasculature and additional layers of tissue with near instantaneous speeds and single-cell precision.

Consumer grade bioprinters do not have the resolution to print microvasculatures required for tissues and organs.  They have a high resolution holographic 3D laser that very similar to 3D lithography.  Hopefully, in the near future, consumer-grade bioprinters will be able to reach the resolution and precision needed to create the tiny blood vessels and Prellis’s process becomes more streamlined so they can make a dent in the global organ shortage.

Here is a gif from their website: https://www.prellisbio.com/ showing the process

 

Dr. Nabeel Cajee

Dr. Cajee is a comprehensive dentist with an interest in advancing implant prosthodontics as a clinician, innovator, and educator. Dr. Cajee is a member of the faculty at the University of the Pacific School of Dentistry in San Francisco and the Dental Ambassador of 3DHeals. He is a recognized Fellow of the International Congress of Oral lmplantologists and the American Academy of Implant Prosthodontics.  He also spent time researching in the Pacific Angiogenesis Laboratory. Dr. Cajee hopes 3D/Bioprinting will unlock creative and deliverable therapies for better patient outcomes.
He highlighted the FDA’s clearance for implantable 3D printed titanium may allow his practices to eliminate inventory of stock implants and parts and create personal products to the patient’s need.  He emphasized dental technology is transitioning from closed systems of technology that is formulaic in process to open systems which may unlock creativity and lower costs; he believes 3D Printing and Bioprinting (in-time) will open up possibilities to advance dental treatments and expand access to care.  He also noted the use of platelet-rich fibrin (PRF) in many dental surgical procedures such as tooth extractions, dental implant surgery, and bone and gum augmentation. Dr. Cajee has documented cases of patient’s not needing to take medications after tooth extractions and implant surgeries due to the accelerating healing properties of PRF.
With PRF,  fibrin, growth factors, and white blood cells are taken from the patient blood.  Since they are from the patients own blood, they are not rejected from the body, rather speed up the healing. The process is the blood is drawn before the procedure normally while the patient is getting numb for the surgery. The blood is then put into a centrifuge at 2700 rpms for 13minutes. After 13 minutes, the blood is separated into three layers – 1) clear liquid or plasma layer 2) red layer rich in red blood cells  3) yellow thick layer which is the PRF layer.
You may visit his talk here: https://www.youtube.com/watch?v=WsyVGYVcl24
Visit his website here: https://www.drcajee.co/about-dr-cajee &  Instagram @drcajee

Tom Anderton

Tom is currently at Squire Patton Boggs, he was formerly General Counsel, Secretary at Zonare Medical Systems and Vice President, Intellectual Property & Legal Affairs at Presidio Pharmaceuticals where he oversaw the legal function at Presidio. Before Presidio, he was the Associate General Counsel and Chief Patent Counsel at Monogram Biosciences, Inc., where he built Monogram’s IP portfolio.  In the talk, he shared the history of patents related to 3D printing and bioprinting, the expiration of important patents in the additive manufacturing industry after 2009 have enabled the expansion of 3D printing into many different technology areas.

In Summary

While 3D printing is well underway for dental and prosthetics, significantly reducing time and costs of production, one of the most exciting developments is the possibility for bioprinting of tissue and organs.  It will be interesting to see how bioprinting further impacts industry, academia, education/global outreach, and patents.

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Junior Capstone Project

final-project-image

This is a summary and reflection about creating a purely mechanical artificial heart in my Junior Capstone class.  Kimberli Graham, Adam Goodwin, Isaac Need, and I finished first place award for our project.

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4D printing

4D Priniting

Self-Assembly and Reaction

4D printing is printing 3D objects that can assembly into another object or preform a reaction to certain conditions. However, the objects need a catalyst to perform their transformation such as water, temperature, light, or other factors. One major player in this movement, who coined the term “4D printing” and created the Self-Assembly Lab, is Skylar Tibbits. He along with Stratsys’s R&D departments and the Connex 3D printer have made important progress in this field. This video clip of a self folding cube by the Self-Assembly Lab shows what can be done within the field of 4D printing. There are more videos from the lab located here.

He saw what nanotechnology was changing in medicine and he is applying the same idea to infrastructure and manufacturing. He isn’t looking to create smart materials that replace designers and engineers, but rather create “programmable materials that build themselves.” It will be interesting to see more to come from this field.

Related Posts

About the Author

Max Murphy is Mechanical Engineering student in his Junior year at California Baptist University. He is interested in the implications of 3D printing or positive manufacturing for mechanical design.  In the summer he is was an intern with Soundfit, one of the companies that is part of the Bay Area Advanced Manufacturing Hub (BAAM), where he is gained hands on experience with a 3D printer and scanner.  He was also an intern with Neodyne Biosciences working with the R&D and Q&A departments.

Biomimicry With 3D Printing of Shapes and Surfaces

Biomimicry

Biomimicry is a approach to design that mimics specific systems or processes found in nature. It has been around a lot longer than 3D printing.  The collage depicts several innovative designs that are directly based on certain properties found in nature.

6BiomimicryExamples

shark_06_1

Sharkskin

For example, Speedo’s Sharkskin swimsuit uses a counter-intuitive approach to reducing drag with a rougher surface that slightly increases turbulence. It’s modeled on the denticles on the surface of a shark’s skin that allow for faster movement than a completely smooth surface. The dimples on a golf ball have similar effect.

The slide show, 7 amazing examples of biomimicry, goes through them in better detail.

Also see the slide show titled “14 Smart Inventions Inspired by Nature: Biomimicry”.

 

Shinkasen Bullet Train

The Shinkansen Bullet Train is another great example of basing designs off things found in nature.  One of the problems with the Shinkansen train was the great loud noise that was created by the friction between the air and the train’s body.
Kingfisher beak and bullet train

“Eiji Nakatsu, an engineer with JR West and a birdwatcher, used his knowledge of the splashless water entry of kingfishers and silent flight of owls to decrease the sound generated by the trains.”
Excerpt from “Shinkansen Train” on Ask Nature

kingfisher_eharrington

Kingfishers move quickly from air, a low-resistance (low drag) medium, to water, a high-resistance (high drag) medium. The kingfisher’s beak provides an almost ideal shape for such an impact. The beak is streamlined, steadily increasing in diameter from its tip to its head. This reduces the impact as the kingfisher essentially wedges its way into the water, allowing the water to flow past the beak rather than being pushed in front of it. Because the train faced the same challenge, moving from low drag open air to high drag air in the tunnel, Nakatsu designed the forefront of the Shinkansen train based on the beak of the kingfisher.
Excerpt from “Shinkansen Train” on Ask Nature

Screenshot (102)

This image is taken from “The three-dimensional shape of serrations at barn owl wings: towards a typical natural serration as a role model for biomimetic applications” by Thomas Bachmann and Hermann Wagner, a great article that goes more in depth into the science behind the serrations on the feather.

Engineers were able to reduce the pantograph’s noise by adding structures to the main part of the pantograph to create many small vortices. This is similar to the way an owl’s primary feathers have serrations that create small vortices instead of one large one.”

Excerpt from “Shinkansen Train” on Ask Nature

The designers used what they observed in nature and applied it to the train to solve their problem.

Scott Sheppard‘s blog post, Eiji Nakatsu: Lecture on Biomimicry as applied to a Japanese Train,

Examples from Janine Benyus

Since writing her book, Biomimicry: Innovation Inspired by Nature, Janine Benyus has become a major figure in the Biomimicry movement by also co-founding the Biomimicry 3.8 and the Biomimicry Institute. Janine Benyus shares a lot of her thoughts and predictions in her one of a few TED Talk which is a great talk if you haven’t seen it already.

There are clearly a lot examples, past and present, of things that got their inspirations from nature.  It has been talked about a lot in regards to 3D printing because 3D printing offers a very high level of customization during the build process.   There are already 3D printed objects based on nature:

and many more.

“Biomimicry is a combination of science, technology, mathematics, and engineering which looks to nature as a teacher to solve modern human design challenges.  3D printing is rapidly changing the way the world interacts with building and making products, and Biomimicry offers a new perspective on this technology.”
Karen McDonald in Biomimicry and 3D Printing

Biomimicry will continue to influence modern 3D printing design principles; not because it is something new, but an approach that has been already applied to many fields.

Related Posts

About the Author

Max Murphy is Mechanical Engineering student in his Junior year at California Baptist University. He is interested in the implications of 3D printing or positive manufacturing for mechanical design.  In the summer he is was an intern with Soundfit, one of the companies that is part of the Bay Area Advanced Manufacturing Hub (BAAM), where he is gained hands on experience with a 3D printer and scanner.  He was also an intern with Neodyne Biosciences working with the R&D and Q&A departments.